Brassinosteroid treatment reveals the importance of xyloglucan transglucosylase/hydrolase (XTH) genes in growth habit determination of twining common bean vines

Data sets for the manuscript "XTH Genes impact Growth Habit Determination of Twining Common Bean Vine Revealed by Brassinosteroid Treatment". Note that there are three treatments: BL (brassinolide, a brassinosteroid), BZ (brassinazole, a brassinosteroid inhibitor), ET (mock treatment, ethanol only). There are also two timepoints. Timpoint A = 3 days after treatment, Timepoint B = 18 days after treatment. (In the Internode 4 elongation dataset, timepoints are noted as 0 (before treatment), 1 (3 days after treatment, Timepoint A), 2 (18 days after treatment, Timepoint B). "Pvul" is short for Phaseolus vulgaris, the latin binomial for the common bean used in this project. Datasets relating to morphology and immunodetection of xyloglucan can be found in the following files:-Internode 4 elongation -G-fiber length -G-fiber thickness -Xyloglucan (presence detected in the G-layer via LM15) Dataset used for the XTH gene tree can be found in the following file: -Pvul_XTH_nucelotide_alignment Datasets relating to RNAsequencing results can be found in the following files: -gene_counts -metadata Differential Gene Expression results: -DEG_results_BL_vs_ET_TimepointA -DEG_results_BL_vs_ET_TimepointB -DEG_results_BZ_vs_ET_TimepointA -DEG_results_BZ_vs_ET_TimepointB DEG_results_BL_vs_BZ_TimepointA DEG_results_BL_vs_BZ_TimepointB (Annotated version of the DEG results are also available for download, but these are not used in the R code and are also presented in the Supporting Files of the article). Phaseolus vulgaris v2.1 genome annotation file to recreate analyses in R can be downloaded from Phytozome. Or downloaded from here as Pvulgaris_annotation.xls. genome.gft file can be found as genome.gft.gz.本数据集配套发表论文《油菜素内酯处理揭示XTH基因调控缠绕型普通菜豆茎蔓生长习性的决定作用》。 注：本实验设置3种处理组：BL（油菜素内酯（brassinolide），属于油菜素内酯类激素）、BZ（油菜素唑（brassinazole），油菜素内酯合成抑制剂）、ET（空白对照，仅以乙醇作为处理介质）。 本研究包含2个采样时间点：时间点A为处理后3天，时间点B为处理后18天。其中在第4节间伸长数据集中，时间点分别标注为0（处理前）、1（处理后3天，即时间点A）、2（处理后18天，即时间点B）。 本研究中，Pvul是普通菜豆（Phaseolus vulgaris）的缩写，该物种为本次实验所用的普通菜豆的拉丁学名。 与形态学及木葡聚糖免疫检测相关的数据集包含以下文件： - 第4节间伸长数据集 - G纤维长度数据集 - G纤维厚度数据集 - 木葡聚糖检测数据集（通过LM15抗体在G层中检测木葡聚糖的存在） 用于XTH基因系统发育树构建的数据集如下： - Pvul_XTH_nucleotide_alignment 与RNA测序结果相关的数据集包含以下文件： - 基因计数矩阵（gene_counts） - 样本元数据（metadata） 差异基因表达分析结果文件如下： - DEG_results_BL_vs_ET_TimepointA - DEG_results_BL_vs_ET_TimepointB - DEG_results_BZ_vs_ET_TimepointA - DEG_results_BZ_vs_ET_TimepointB - DEG_results_BL_vs_BZ_TimepointA - DEG_results_BL_vs_BZ_TimepointB 此外，差异基因表达结果的注释版可供下载，但该版本未在R代码分析中使用，仅随论文支持材料一同发布。 可从Phytozome数据库下载用于R语言重现分析的普通菜豆v2.1版本基因组注释文件，也可通过本数据集获取该文件，文件名为Pvulgaris_annotation.xls。基因组GFT格式文件可通过genome.gft.gz获取。