Luecken Cite-seq human bone marrow 2021

Dataset published by Luecken et al. 2021 which contains data from human bone marrow measured through joint profiling of single-nucleus RNA and Antibody-Derived Tags (ADTs) using the 10X 3' Single-Cell Gene Expression kit with Feature Barcoding in combination with the BioLegend TotalSeq B Universal Human Panel v1.0.<b>File Description</b><i>cite_filtered.h5mu</i>: Filtered barcode by feature MuData object of concatenated donor’s raw counts from filtered barcodes. This is built from the Cell Ranger output and contains the RNA and ADT ("prot" key) data.<i>cite_raw.h5mu</i>: Unfiltered barcode by feature MuData object of concatenated donor's raw counts extracted by Cell Ranger. It contains the RNA and ADT ("prot" key) data.<i>cite_preprocessed.h5ad</i>: Fully processed AnnData object used in annotation chapter.<b>Citation</b>Luecken, M. D. <i>et al.</i> A sandbox for prediction and integration of DNA, RNA, and proteins in single cells. Thirty-fifth Conference on Neural Information Processing Systems Datasets and Benchmarks Track (2021).<br><b>Original data link</b>https://openproblems.bio/neurips_docs/data/dataset/

本数据集由Luecken等人于2021年发布，包含通过单细胞核RNA与抗体衍生标签（Antibody-Derived Tags, ADTs）联合检测分析得到的人类骨髓样本数据。实验采用搭载特征条形码（Feature Barcoding）的10X 3'单细胞基因表达试剂盒（10X 3' Single-Cell Gene Expression kit），并结合BioLegend TotalSeq B通用人类组合v1.0（BioLegend TotalSeq B Universal Human Panel v1.0）完成检测。<b>文件说明</b><i>cite_filtered.h5mu</i>：经过滤的条形码-特征矩阵MuData对象，整合了来自过滤后条形码的供体原始计数数据。该对象基于Cell Ranger输出结果构建，包含RNA与ADT（键名为"prot"）数据。<i>cite_raw.h5mu</i>：未经过滤的条形码-特征矩阵MuData对象，包含由Cell Ranger提取的供体原始计数整合数据，同样涵盖RNA与ADT（键名为"prot"）数据。<i>cite_preprocessed.h5ad</i>：用于注释章节的全预处理完成的AnnData对象。<b>引用信息</b>Luecken, M. D. 等人. 单细胞中DNA、RNA与蛋白质的预测及整合沙箱. 第三十五届神经信息处理系统大会数据集与基准赛道 (2021).<br><b>原始数据链接</b>https://openproblems.bio/neurips_docs/data/dataset/