Comparative Morphometric Analysis of Corneal Endothelium Before and After Transplantation Using Voronoi Segmentation

This study reports a novel comparative ex vivo and in vivo analysis of the morphometric changes in corneal endothelium pre- and post-transplantation.Images of ex vivo corneal donor tissue pre-transplantation were compared with post-transplant in vivo confocal microscope (IVCM) images of the same tissue. These images were calibrated and analysed using Voronoi segmentation evaluating cell size, shape, and phase. Statistics collected: Donor Characteristics: Donor age (years), Death - storage duration (hours), Organ culture storage duration (days)Pre- and Post-Transplant: Days between pre- and post-transplant, Endothelial cell density (cells/mm2), Cell area (µm2), Area squares (%), Area pentagons (%), Area hexagons (%), Area heptagons (%), Area octagons (%), Area nonagons (%), Number of sides, Number of shapes, Hexagonal regularity (%), Coefficient of variation in cell area (%), Coefficient of variation in number of sides (%), Shape Index, Topological charge, Number of cells本研究报道了一项新颖的离体（ex vivo）与体内（in vivo）对比分析，旨在探究角膜内皮细胞移植前后的形态计量学变化。 将移植前离体角膜供体组织的图像，与同一组织移植后获取的体内共聚焦显微镜（in vivo confocal microscope, IVCM）图像进行对比。 上述图像均经过校准，并采用沃罗诺伊（Voronoi）分割算法进行分析，以评估细胞尺寸、形态及相位特征。 收集的统计指标如下： 供体特征：供体年龄（岁）、死亡-保存时长（小时）、器官培养保存时长（天） 移植前后相关指标：移植前后间隔天数、内皮细胞密度（cells/mm²）、细胞面积（µm²）、正方形区域占比（%）、五边形区域占比（%）、六边形区域占比（%）、七边形区域占比（%）、八边形区域占比（%）、九边形区域占比（%）、边数、形状数量、六边形规整度（%）、细胞面积变异系数（%）、边数变异系数（%）、形状指数、拓扑电荷、细胞总数