Lipid mediator abundance in skeletal muscle of BALB/C mouse with and without C26-induced cancer cachexia

This study investigated whether cancer affected the muscle lipid mediator abundance. Male BALB/c mice were injected with PBS (CON) or C26 colon carcinoma cells (C26) (n = 5/group). Mice were sacrificed 18 days upon PBS/tumor cell injection.
The relative abundance of lipid mediators was analyzed in the vastus medialis muscle (~15mg; part of m. Quadriceps). Lipid extraction and the LC-MS/MS–based lipidomics was performed by the KU Leuven Lipometrix core facility. More detail is provided by Dalle, S., Hiroux, C., & Koppo, K. (2024). Endocannabinoid remodeling in murine cachexic muscle associates with catabolic and metabolic regulation. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1870(5), 167179. https://doi.org/10.1016/j.bbadis.2024.167179

本研究旨在探究癌症是否会对肌肉脂质介质的丰度产生影响。将雄性BALB/c小鼠分别注射磷酸盐缓冲液（PBS，CON，对照组）或C26结肠癌细胞（C26组），每组n=5。于注射PBS/肿瘤细胞后的第18天处死小鼠。
对股内侧肌（约15mg，为股四头肌（m. Quadriceps）的一部分）中的脂质介质相对丰度进行分析。脂质提取及基于液相色谱-串联质谱（LC-MS/MS）的脂质组学分析由鲁汶大学（KU Leuven）脂质组核心设施（Lipometrix core facility）完成。详细研究细节可参见Dalle S、Hiroux C与Koppo K于2024年发表的研究论文：《小鼠恶病质肌肉中的内源性大麻素重塑与分解代谢及代谢调控相关》，刊载于《生物化学与生物物理学报（BBA）-疾病分子基础》，第1870卷第5期，文章编号为167179，DOI：https://doi.org/10.1016/j.bbadis.2024.167179