Data underlying the research on particle manipulation using hydrodynamic forcing. This dataset consist of the experimental data of an arbitrarily selected pollen grain trapping in a Hele-Shaw cell.

The reseach objective is to present a microfluidic approach to achieve the dynamic control of particle pathlines within a flow through microfluidic device. Our approach is general, and not limited to one particular kind of particle. The flow is used as gentle tweezers to move particles within the flow cell and the approach is particularly suited to biological sample. To demonstrate this, an arbitrary pollen grain is selected and trapped in the flow cell while the other pollen grains continue to flow. Our approach combines three key aspects: the design of a flow-through microfluidic flow cell with the ability to manipulate the streamlines of the flow, an optimization procedure to find a priori optimal particle path-lines, and a Proportion-Integral-Derivative-based (PID) feedback controller to provide real time control over the particle manipulations. The experimental raw images were recorded with a sCMOS camera (PCO) with a pixel pitch of 6.5 μm. The camera was mounted on a microscope (Nikon Eclipse Ti) with a 1x objective. The acquisition frequency was 5 Hz corresponding to an average in-plane displacement of 4-6 pixels between two consecutive recordings. An additional sCMOS camera (PCO) with 3x zoom lens is used for the visualization of the trapped pollen. The zip file contains the raw images and the MATLAB script used to do an experiment of arbitarily selecting a pollen grain and trapping it by only using the hydrodynamic forcing in a Hele-Shaw cell.本研究的目标为提出一种微流控方法，以实现微流控装置内流场中粒子迹线的动态调控。本方法具备普适性，不受限于单一类型的粒子。该流场可作为温和的流体操控镊子，在流室中实现粒子的移动，且该方法尤其适用于生物样本。为验证该方法的可行性，研究选取任意一粒花粉粒将其捕获于流室中，其余花粉粒则随流持续运动。本方法整合了三项核心技术环节：其一为可调控流场流线的流通式微流控流室设计，其二为用于获取先验最优粒子迹线的优化流程，其三为基于比例-积分-微分（Proportion-Integral-Derivative，PID）反馈控制器，以实现粒子操控的实时调控。实验原始图像由像素间距为6.5μm的sCMOS相机（sCMOS camera，PCO品牌）采集，该相机搭载于配备1×物镜的尼康（Nikon）Eclipse Ti显微镜上。采集频率设置为5Hz，对应两次连续采集之间的平面平均位移为4~6个像素。额外配备了搭载3×变焦镜头的PCO品牌sCMOS相机（sCMOS camera），用于可视化观察被捕获的花粉粒。本压缩包包含实验原始图像以及用于开展如下实验的MATLAB脚本：在赫勒-肖（Hele-Shaw）流室中，仅通过流体动力操控，任意选取并捕获单粒花粉粒。