Assembled and annotated contigs from the metagenomic sequencing data generated from the redoxcline of the central western gyre of the Black Sea (UKR11/002)

The overarching goal of the cruise was to investigate the biology and biogeochemistry of the central Black Sea with special consideration of the processes at the pelagic redoxcline. Water samples were collected with free-flow niskin bottles connected to a CTD rosette. Samples for the assessment of the metatranscriptome were fixed in situ and ribosomal RNA was depleted before sequencing. Metagenome and -transcriptome reads were generated with Illumina HiSeq 2500. Community composition was derived from the V3-V4 fragment of the 16S rDNA and 16S rRNA gene sequence of the same sample. DNA and RNA were extracted using AllPrep DNA/RNA Kit from QIAGEN (Hilden, Germany). Samples for DNA sequencing where stored and samples for RNA sequencing were reverse transcribed with Multiscribe RT (Thermo Fisher Scientific, Waltham, MA, USA) after DNase treatment with TURBO-DNA-free Kit (Thermo Fisher Scientific, Waltham, MA, USA). Sequencing was done with SciLifeLab (Metagenome; Illumina HiSeq 2500; Stockholm, Schweden), Fasteris (Metatranscriptome, Illumina HiSeq 2500, Plain-Les-Ouates, Switzerland), LGC Genomics (Amplicon sequencing, Illumina MiSeq V3, Berlin, Germany). This data set includes the assembled contigs from the metagenomic data and their annotation.

本航次的核心目标为探究黑海中部海域的生物学与生物地球化学特征，重点关注远洋氧化还原跃层(pelagic redoxcline)处的相关过程。研究采用连接至CTD采水架(CTD rosette)的自由流动式尼斯金采水瓶采集海水样本。用于元转录组(metatranscriptome)检测的样本采用原位固定，测序前先去除核糖体RNA(ribosomal RNA)。宏基因组(metagenome)与元转录组测序读段通过Illumina HiSeq 2500平台生成。群落组成通过同一样本的16S核糖体DNA(16S rDNA)与16S核糖体RNA(16S rRNA)基因序列的V3-V4可变区分析得到。脱氧核糖核酸(DNA)与核糖核酸(RNA)采用德国希尔德市QIAGEN公司的AllPrep DNA/RNA试剂盒提取。DNA测序样本经保存后，RNA测序样本先采用美国马萨诸塞州沃尔瑟姆市Thermo Fisher Scientific公司的TURBO-DNA-free试剂盒进行脱氧核糖核酸酶(DNase)处理，随后通过Multiscribe RT完成反转录。测序工作分别由三家机构完成：瑞典斯德哥尔摩的SciLifeLab（承担宏基因组测序，平台为Illumina HiSeq 2500）、瑞士普莱讷欧特的Fasteris（承担元转录组测序，平台为Illumina HiSeq 2500）以及德国柏林的LGC Genomics（承担扩增子测序，平台为Illumina MiSeq V3）。本数据集包含宏基因组数据经组装得到的重叠群(contigs)及其注释信息。