Comparing the overall Kantorovich-Rubinstein distance between truncated and full-length sequencing data.

Quality controlled 454 sequencing data longer than 120 bp was truncated and processed using the pplacer pipeline (Fig. 2). Merged placements from the truncated data for all 60 samples were compared to the same full-length (454 and Illumina) data by calculating the tree-length normalized KR distances between all pair-wise combinations of the three data sets.对经过质量控制、长度超过120碱基对（base pair, bp）的454测序（454 sequencing）数据进行截短处理，并借助pplacer流程（pplacer pipeline）完成分析（见图2）。针对全部60个样本的截短数据所得到的合并序列系统发育放置结果，与全长（454与Illumina）测序数据进行对比，通过计算该三个数据集所有两两组合之间的树长归一化KR距离，完成对比分析。