Order parameters (2SCD) for deuterium labelled POPC incorporated into nuclear envelope remnants and model membranes (MLVs).

Cholesterol/cholesteryl ester and phospholipid concentrations were determined by colorimetry. Cholesterol contents are expressed as molar percentages relative to total phospholipids. Nuclear envelope remnant order parameter was calculated from the 2H NMR spectrum of labelled nuclear envelope remnants acquired post-equilibrium (Tfin). POPC MLVs containing 0, 5, 15 or 30 mol% cholesterol values are taken from Garnier-Lhomme et al.[34]. Deuterium spectra of labelled nuclear envelope remnants and MLVs were acquired at 10°C after equilibration at 40°C.