Order parameters (2SCD) for deuterium labelled POPC incorporated into nuclear envelope remnants and model membranes (MLVs).

Cholesterol/cholesteryl ester and phospholipid concentrations were determined by colorimetry. Cholesterol contents are expressed as molar percentages relative to total phospholipids. Nuclear envelope remnant order parameter was calculated from the 2H NMR spectrum of labelled nuclear envelope remnants acquired post-equilibrium (Tfin). POPC MLVs containing 0, 5, 15 or 30 mol% cholesterol values are taken from Garnier-Lhomme et al.[34]. Deuterium spectra of labelled nuclear envelope remnants and MLVs were acquired at 10°C after equilibration at 40°C.采用比色法（colorimetry）测定胆固醇/胆固醇酯（cholesterol/cholesteryl ester）与磷脂（phospholipid）的浓度。胆固醇含量以相对于总磷脂（total phospholipids）的摩尔百分比（molar percentages）表示。核被膜残体（nuclear envelope remnant）的有序参数，根据平衡后（Tfin）采集得到的标记型核被膜残体的氘核磁共振（2H NMR）谱图计算得到。含0、5、15或30 mol%胆固醇的1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）多层囊泡（MLVs）的数据取自Garnier-Lhomme等人[34]的研究。标记型核被膜残体与多层囊泡的氘核磁共振谱均先在40℃下平衡，随后在10℃下采集。