Supplementary Figure 4. Depletion of PMNs does not induce myopathy

(A) Representative flow cytometric plots of CD11b+Gr1+ cells isolated from heart of rIgG- or αLy6G-treated mice. (B) Percentage of body weight change. (C-D) MW/TL (C), and percentage change of MW/TL (D) of rIgG- or αLy6G-treated mice (n=5). (E-F) HW/TL (E) and percentage change of HW/TL (F) of rIgG- or αLy6G-treated mice (n=5). (G) Representative echocardiographic images (M-mode) showing left ventricular internal diameter end diastole and end systole. Cardiac functions including the percentage of ejection fraction (H-I), cardiac output (J-K), and stroke volume (L-M) of rIgG- or αLy6G-treated mice (n=5). (N) Representative Picrosirius Red staining of cardiac tissue from control or long-term (D4w+4w) DOX treated mice (n=4-5). (O) Quantification of fibrotic area normalized to cardiomyocyte area of control or DOX treated mice (n=4-5).