Characterization of the regulatory network of linc1393 in the maintenance of pluripotency provides the principles for lncRNA targets prediction [RNA-seq]

In the study, we screened the nuclear lncRNAs in mESCs, and demonstrate that linc1393 maintains mESCs pluripotency by recruiting SET1A nearby its binding sites, to establish H3K4me3 and activate the expression of specific pluripotent genes. Furthermore, we developed a computational framework, LncTargeter, to predict the binding sites and the target genes of a given lncRNA based on the XGBoost model. Overall design: The mechanism of linc1393 on the maintenance of pluripotency and development of LncTargeter RNA-seq in mESCs