Data Sets of ESR1 Expression in HESC cultures and ESR1 and PR levels in Wistar rat's endometrium when treated with Adansonia digitata root bark fractions.

Adansonia digitata Linn root bark extract alters oestrogen and progesterone activities in the female Wistar rat. We hypothesized that components of the extract may act through the regulation of oestrogen and progesterone transduction. We obtained fractions from Aqueous methanol extract of Adansonia digitata root bark through Column chromatography with different solvents; acetyl acetate, methanol, and distilled water, to yield acetyl acetate-methanol fraction (AAMF); Aqueous methanol fraction (AQMF); and Aqueous fraction (AQF). The fractions were investigated for their effect on the gene expression of oestrogen receptor alpha (ESR1) in Human endometrial stromal cell (HESC) line (Data 1).
The effect of the acetyl acetate-methanol fraction of the extract on the location and levels of ESR1 and progesterone receptor alpha-beta (PR) in the mature virgin female Wistar rat's uterus was also investigated (Data 2 - 6).
The results revealed that AAMF at 50 – 500 µg /ml downregulates ESR1 gene expression in HESC cultures. In the Wistar rat's uterus, AAMF at 150 - 300 mg/kg bodyweight caused a reduction in membrane-located ESR1 levels in the endometrial glands and epithelium, but increased PR levels in the endometrial stroma.

猴面包树（Adansonia digitata Linn）根皮提取物可改变雌性Wistar大鼠体内的雌激素（oestrogen）与孕酮（progesterone）活性。本研究推测，该提取物的活性成分可能通过调控雌激素与孕酮的信号转导过程发挥作用。我们采用柱层析法（column chromatography），以乙酸乙酯（acetyl acetate）、甲醇（methanol）及蒸馏水为洗脱溶剂，从猴面包树根皮的水甲醇提取物中分离得到三个组分：乙酸乙酯-甲醇组分（AAMF）、水甲醇组分（AQMF）以及水组分（AQF）。
我们检测了各组分对人子宫内膜基质细胞（Human endometrial stromal cell, HESC）系中雌激素受体α（oestrogen receptor alpha, ESR1）基因表达的影响（数据集1）。
本研究还探究了该提取物的乙酸乙酯-甲醇组分对成熟未交配雌性Wistar大鼠子宫内ESR1与孕酮受体αβ（progesterone receptor alpha-beta, PR）的定位及表达水平的影响（数据集2至6）。
实验结果显示，浓度为50~500 μg/ml的AAMF可下调HESC培养体系中ESR1的基因表达水平。在Wistar大鼠子宫中，剂量为150~300 mg/kg体重的AAMF可降低子宫内膜腺体与上皮细胞膜定位的ESR1水平，但可升高子宫内膜基质内的PR表达水平。