Immunological responses induced in C57Bl/6 following 3 vaccinations with CS/D adjuvanted with GLA/SE or 3M051.

Groups of 15 C57Bl/6 mice were immunized three times, 3 weeks apart, with 2.5 µg of CSP in the indicated adjuvant. Sera were analyzed 2 weeks after the last dose. A, ELISA titers of mice measured against CS/D (left) and NANP peptide (right). Two high responding mice from the GLA/SE group were included in statistical analysis are outside the scale of the NANP graph. B, Relative IgG1 vs. IgG2c responses against the C term protein (left) or NANP peptide (right) measured by Luminex at 1∶2000 serum dilution. C, Percentage of total Cytokine+CD44+CD4+ T lymphocytes, extracted from vaccinated C57Bl/6 mice and stimulated with CS/D, stained for intracellular IL-2 (left) or IFN-γ (right). Lines are mean with SEM.