Metadata record for the article: MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer

<b>Summary</b><br> This metadata record provides details of the data supporting the claims of the related article: “MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer”. The related study aimed to determine how triple negative breast cancer (TNBC) suppresses anti-tumour macrophages by using microRNA-200c (miR-200c), a powerful repressor of epithelial-to-mesenchymal transition (EMT), to drive mesenchymal-like mouse mammary carcinoma and human TNBC cells towards a more epithelial state. Type of data: mRNA sequencing Subject of data: <i>Mus musculus</i>; Eukaryotic cell lines; antibodies Sample size: When appropriate, tissue culture experiments are the mean of three separate experiments conducted in triplicate to decuple. For in vivo experiments, animal numbers were calculated at 80% power to the expected difference (based on published studies) at P &lt; 0.05 (two-tailed). Population characteristics: 6-8 week old female FVB/NJ mice (IMSR Cat# JAX:001800, RRID:IMSR_JAX:001800) <b>Data access</b> The mRNA sequencing data are openly available in the <i>Gene Expression Omnibus</i> via the following accession: https://identifiers.org/geo:GSE151320. The majority of the other data files generated as part of the related study are openly available as part of this data record. A comprehensive list of which data underlie which element of the related article is available as part of this data record in the file ‘Williams_et_al_2021_underlying_data_list.xlsx’. Other data used in the related study are already openly available in various repositories, and access details are also given in the file mentioned above. Data supporting generation of Met-1 TripZ-200c and Met-1 TripZ-EV cells are contained in the files ‘Cell sort RPF- 011818.pdf’ and ‘Cell sort RFP+ 020918.pdf’. <b>Corresponding author(s) for this study</b> Jennifer K Richer, Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO USA. [e-mail]. <br> <b>Study approval </b> All animal experiments were performed in accordance with international, national and institutional guidelines for humane research under a protocol (#00407) approved by the University of Colorado Institutional Animal Care and Use Committee (IACUC).

**数据集概述**
本元数据记录详细阐述了支撑相关论文《MicroRNA-200c恢复揭示细胞因子谱以增强乳腺癌中M1巨噬细胞极化》核心论点的实验数据。本项相关研究旨在阐明三阴性乳腺癌（triple negative breast cancer, TNBC）如何通过微小RNA-200c（microRNA-200c, miR-200c）——一种强效的上皮间质转化（epithelial-to-mesenchymal transition, EMT）抑制因子——将间质样小鼠乳腺癌细胞与人类三阴性乳腺癌细胞重编程为更具上皮表型的细胞，从而抑制抗肿瘤巨噬细胞的功能。
数据类型：mRNA测序（mRNA sequencing）
数据对象：小家鼠（Mus musculus）；真核细胞系；抗体
样本量：适当时，组织培养实验的结果为3次独立重复实验的均值，每次独立实验包含3至10个技术重复；体内实验的动物数量则基于已发表研究的预期差异，通过功效分析确定为80%检验效能，显著性阈值设为P<0.05（双侧检验）。
实验群体特征：6~8周龄雌性FVB/NJ小鼠（国际小鼠资源库货号：IMSR Cat# JAX:001800，RRID:IMSR_JAX:001800）
**数据获取**
mRNA测序数据可通过以下登录号在基因表达综合数据库（Gene Expression Omnibus）公开获取：https://identifiers.org/geo:GSE151320。本项研究生成的其余多数数据文件可在本数据集记录中公开获取。本数据集记录中附带的"Williams_et_al_2021_underlying_data_list.xlsx"文件，完整列出了支撑相关论文各部分内容的实验数据清单。本研究使用的其余公开数据已存储于各类公共数据库，获取方式详见上述文件。用于支撑Met-1 TripZ-200c与Met-1 TripZ-EV细胞系构建的数据，分别存储于"Cell sort RPF-011818.pdf"与"Cell sort RFP+ 020918.pdf"文件中。
**本研究通讯作者**
詹妮弗·K·里彻（Jennifer K Richer），美国科罗拉多州奥罗拉市科罗拉多安舒茨医学校园病理学系，邮箱：[e-mail]。
**实验伦理审批**
所有动物实验均遵循国际、国家及机构的人道研究指南开展，相关实验方案（编号：00407）已获科罗拉多大学实验动物护理与使用委员会（Institutional Animal Care and Use Committee, IACUC）批准。