Summary of imprinted candidates identified in rice embryo and endosperm by transcriptome analyses and those confirmed by PCR.

1: Three embryo genes that met significance and four additional genes with suggestive evidence were analysed. Only one gene proved to be imprinted and it arose from the four additional genes analysed (Figure 1E). This gene is also imprinted in the endosperm.2: Genes with ≥90% bias were tested.3: Including 1 transcript from an intergenic region and five transcripts from intronic regions.4: Maternal and paternal biased transcripts of different structure were predicted to originate from these genes as a result of novel alternative splicing. One locus was confirmed to show parent of origin-biased splicing (Figure 2 and Figure S5).1: 本研究对3个达到显著性检验标准的胚胎基因，以及4个仅获得提示性证据的额外基因进行了分析。最终仅1个基因被证实为印迹基因（imprinted），且该基因属于上述4个额外分析的基因范畴（图1E）。该基因在胚乳（endosperm）中同样表现出印迹特性。 2: 对亲本起源表达偏倚程度≥90%的基因开展检测验证。 3: 本次分析涵盖1个来自基因间区（intergenic region）的转录本（transcript）及5个来自内含子区域（intronic region）的转录本。 4: 经预测，源自这些基因的母本偏倚转录本与父本偏倚转录本结构存在差异，该现象由新型可变剪接（alternative splicing）事件介导。另有1个基因座（locus）被证实存在亲本起源偏倚的可变剪接现象（图2及补充图S5）。