Genome-wide mapping of regulatory regions in seven domestic cat tissues [ChIP-seq]

Histone modifications and CTCF mark the locations of genomic regulatory regions -- including promoters, enhancers, and insulators -- and have not been previously annotated for the domestic cat genome. Understanding where non-coding sequence variants fall in relation to regulatory regions is vital for determining their impact on gene function and their ability to cause disease. The addition of replicated feline ChIP-seq data from multiple tissues will aid in interpretation of non-coding variants, furthering characterization of genetic diseases and genetic test development. Overall design: Genomic DNA was extracted from seven tissues collected from adult male domestic shorthair cats (two biological replicates for each tissue), and ChIP-seq was performed with antibodies to three histone modifications (H3K27ac, H3K4me3, H3K27me3) and CTCF on all tissues. As a control, input DNA was sequenced for each tissue without the inclusion of an antibody. ENCODE quality metrics (NRF, PBC1, PBC2, NSC, and RSC) were calculated and included in the provided sample metadata.