Higher colonic proliferation and tumor prevalence amongst azoxymethane treated A/J mice receiving fecal microbiota transplants from old mice compared to recipients of young mice [RNA-Seq]

Gut microbial dysbiosis can play a causal role of in colorectal cancer. Gut microbiota chnages with age and becomes moer pro-inflammatory. We sought to determine whether microbiota from Old donors promotes more tumor formation in recipients than meterial from young donors. Overall design: We conducted fecal material transplants (FMT) from young (~6 wk) and old (~72 wk) donor mice into young (8 wk) recipient mice that were pre-treated with antibiotics. After initiating tumorigenesis with azoxymethane, recipients were maintained for 19 weeks during which time they received monthly FMT boosters.