Torsten Wittmann, Gary M. Bokoch, Clare M. Waterman-Storer (2011) CIL:11841, Potorous tridactylus, epithelial cell. CIL. Dataset

Microtubules in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 4, B and D of associated paper (J Cell Biol, 161:845-851, 2003).

在表达 Rac1(Q61L) 的 PtK1 细胞中注射 Pak 抑制剂片段 PBD/ID(H83L) 的微管。微管未表现出逆行流动，且净增长极小。在倒置显微镜（型号 TE300；尼康）上获取了正置荧光图像系列，该显微镜配备有电子控制快门、滤色轮和 14 位冷却 CCD 相机（Orca II；Hamamatsu 公司），并由 MetaMorph® 软件（Universal Imaging Corp.）控制，使用 60/1.4 NA Plan Apo 物镜（尼康）以 5 秒间隔拍摄图像。图像每 5 秒拍摄一次，以 15 帧/秒的速度回放。静止图像请参见相关论文（J Cell Biol，161:845-851，2003）的图 4，B 和 D。