Dynamics of bacterial operons during genome-wide stresses are influenced by premature terminations and internal promoters

Bacterial gene networks have many operons, each coordinating a few genes. In Escherichia coli, half of the operons have internal promoters regulating downstream genes independently from upstream genes. We studied their role during genome-wide stresses targeting key transcription regulators, RNAP, and gyrase. We show that the operons' stress response dynamics are highly influenced by premature terminations of transcription elongation and internal promoters (specifically, their numbers, positioning, and responsiveness). Moreover, premature terminations are influenced by positive supercoiling buildup, collisions between elongating and promoter-bound RNAPs, and sequences commonly found at 3’ ends of RNA. We report the same findings in E. coli cells subject to other stresses and in the evolutionarily distant Bacillus subtilis, Corynebacterium glutamicum, and Helicobacter pylori. Our results suggest that the strength, number, and positioning of internal promoters in operons are subject t..., , , # Dynamics of bacterial operons during genome-wide stresses are influenced by premature terminations and internal promoters

Dryad DOI: [https://doi.org/10.5061/dryad.zgmsbccks](https://doi.org/10.5061/dryad.zgmsbccks)

This READ ME.txt file was generated on 10-03-2024 by Rahul Jagadeesan.

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细菌基因网络包含大量操纵子（operon），每个操纵子可协调若干基因的协同表达。在大肠杆菌（Escherichia coli）中，有半数操纵子携带内部启动子，能够独立调控下游基因的转录，不受上游基因序列的影响。本研究针对全基因组范围内靶向关键转录调控因子、RNA聚合酶（RNAP）以及旋转酶（gyrase）的胁迫条件，探究了此类内部启动子的生物学功能。研究结果表明，操纵子的胁迫响应动态极大程度上受转录延伸提前终止事件与内部启动子（具体包括其数量、位置与响应活性）的影响。此外，转录提前终止还受到正超螺旋积累、延伸中RNA聚合酶与启动子结合态RNA聚合酶之间的碰撞，以及RNA 3'端常见序列特征的调控。我们在经受其他胁迫条件的大肠杆菌细胞，以及进化亲缘关系较远的枯草芽孢杆菌（Bacillus subtilis）、谷氨酸棒状杆菌（Corynebacterium glutamicum）和幽门螺杆菌（Helicobacter pylori）中均得到了一致的实验发现。本研究结果提示，操纵子内部启动子的强度、数量与位置均受到……

# 全基因组胁迫下细菌操纵子的动态变化受转录提前终止与内部启动子调控

Dryad数字对象标识符：https://doi.org/10.5061/dryad.zgmsbccks

本READ ME.txt文件生成于2024年10月3日，作者为拉胡尔·贾加迪桑（Rahul Jagadeesan）。

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