Investigate the effect of depletion of Mettl14 or Ythdf2 on RNA stability in primary cultured cortical neurons

Across the transcriptome, m6A-modified transcripts showed increased relative stability in the Mettl14 cKO condition , and the number of m6A sites affected the stability. The stability of transcripts with m6A only in the CDS changed slightly less, while the stability of transcripts with m6A only in the 3' UTR or with m6A in both regions increased more significantly, suggesting that m6A in the 3' UTR has a more pronounced effect on mRNA stability. Unexpectedly, m6A-modified transcripts showed only a slight stability change in the DF2 cKO condition. Overall design: To indentify the stability of each gene from neurone, cultured primary cortical neurons from WT and Mettl14 cKO mice, or WT and Ythdf2 cKO mice were treated with Actinomycin D, and RNA-seq was performed at 0 and 5 hours to calculate the RNA stability of each transcript.