Zebrafish as a model for muscle ageing research and exosomal communication during muscle regeneration

Research on ageing, particularly skeletal muscle ageing, is vital for extending health span and addressing related diseases. This thesis confirms the suitability of zebrafish as a model for muscle ageing research, revealing ageing hallmarks comparable to those observed in mammals. Additionally, the project established a new live imaging system for adult zebrafish, which enhances the capacity to examine cellular interactions in the adulthood stage, a previously underexplored aspect in this species. The research also reveals the real-time exosomal communication between myocytes, macrophages, and muscle stem cells during regeneration. The functional analysis reveals that stem cell proliferation was impaired when exosome was inhibited.