Examining marine assemblages across an inverse salinity gradient

Salinity gradients and fluctuations can create a natural ecological filter, with few species tolerating salinity above 50 practical salinity units (PSU). We investigated how an inverse salinity gradient affected marine community diversity and composition in Shark Bay, a remote hypersaline coastal embayment in Western Australia. We used 16S(fish), 18S, and 16S(Bacteria) eDNA metabarcoding assays to analyse genetic sequences collected from 220 surface water samples and 216 benthic roller samples. The 44 survey sites transcended a salinity gradient of 40.1 PSU in the Eastern Gulf to 76.0 PSU in Hamelin Pool. 24,011,345 eDNA sequencing reads were detected from 5,575 operational taxonomic units (OTUs). The number of Families and OTUs, and OTUs within key Phyla, all decreased with increasing salinity, the most dramatic changes occurring over a narrow salinity range (50 - 60 PSU). However, the relative proportion of Families from each Phyla contributing to the total assemblage within each sali..., , # Data from: Examining marine assemblages across an inverse salinity gradient Dataset DOI: [10.5061/dryad.xpnvx0kt4](10.5061/dryad.xpnvx0kt4) ## Description of the data and file structure The three eDNA files and the metadata file stored here were collected from Shark Bay, Western Australia eDNA data was collected from 220 surface water samples and 216 benthic roller samples taken from 44 sites across Eastern Shark Bay, Western Australia. Five replicate water and roller samples were collected at each of the 44 sites, with the exception of four roller sites, where four, rather than five, replicates were taken. Sites were spread across four habitats (sand, seagrass, low relief stromatolites, and high relief stromatolites). ### Files and variables #### Files and variables Files: RAW_16S_Chordata.csv, RAW_18S.xlsx, RAW_16S_Bacteria.xlsx **Description:** Variables * Taxonomy allocated to that OTU (domain, phylum, class, order, family, genus, species) * OTU: Operational Taxonomic U..., ,盐度梯度及其波动可形成天然生态过滤屏障，仅有极少数物种能够耐受盐度高于50实用盐度单位（Practical Salinity Units, PSU）的水环境。本研究以澳大利亚西部偏远的超盐度近岸海湾——鲨鱼湾（Shark Bay）为研究区域，探究了逆向盐度梯度对海洋群落多样性与组成的影响。研究采用16S（鱼类）、18S及16S（细菌）的环境DNA（environmental DNA, eDNA）元条形码测序分析技术，对220份表层水样与216份底栖滚筒采样样本的遗传序列进行分析。44个调查样点的盐度跨度为东湾的40.1 PSU至哈梅林池的76.0 PSU。本研究共检测到来自5575个操作分类单元（Operational Taxonomic Unit, OTU）的24011345条eDNA测序读段。科与OTU的数量，以及关键门类下的OTU数量，均随盐度升高而降低，其中最显著的群落变化发生在50~60 PSU的狭窄盐度区间内。然而，各门类下的科级类群在各盐度梯度下总群落组成中的相对占比……，，# 数据来源：《跨逆向盐度梯度的海洋群落研究》 数据集DOI：[10.5061/dryad.xpnvx0kt4](10.5061/dryad.xpnvx0kt4) ## 数据与文件结构说明 本数据集存储的三份eDNA数据文件与一份元数据文件均采集自澳大利亚西部的鲨鱼湾。 eDNA数据采集自西澳东鲨鱼湾44个样点的220份表层水样与216份底栖滚筒采样样本。除4个滚筒采样点仅采集4次重复样本外，其余44个样点均采集5次表层水与滚筒采样重复样本。样点分布于4类生境：沙质底、海草床、低凸起叠层石与高凸起叠层石。 ### 文件与变量 #### 文件与变量 文件列表：RAW_16S_Chordata.csv、RAW_18S.xlsx、RAW_16S_Bacteria.xlsx **说明：** 变量信息包括： * 该OTU的分类学注释（域、门、纲、目、科、属、种） * OTU：操作分类单元（Operational Taxonomic Unit）……