Detection of misfolded SOD1 in various structures and regions by immunohistochemistry.

Relative abundance of DSE antibody (10E11C11) staining in various regions and structures of patient spinal cord sections (N = 5 normal control; N = 5 SOD1-FALS; N = 3 non-SOD1 FALS including R521C-FUS; N = 20 non-SOD1 SALS). Neuronal cytoplasmic inclusions, axonal swellings and axons are sub-structures within the ventral grey matter. Positive staining in corticospinal tract, other tracts and motor roots was in axons. –, no staining; +, some staining; ++, moderate staining; +++, abundant staining.患者脊髓切片各区域及结构中DSE抗体（10E11C11）染色的相对丰度。本研究共纳入正常对照5例（N=5）、SOD1相关家族性肌萎缩侧索硬化（SOD1-FALS）5例、携带R521C-FUS突变的非SOD1家族性肌萎缩侧索硬化（non-SOD1 FALS）3例，以及非SOD1散发性肌萎缩侧索硬化（non-SOD1 SALS）20例。腹侧灰质内的亚结构包括神经元胞质包涵体、轴突肿胀及轴突。皮质脊髓束、其余纤维束及运动神经根的阳性染色均定位于轴突。其中"–"代表无染色，"+"代表少量染色，"++"代表中等强度染色，"+++"代表大量染色。