Expression analysis of wt and maternal Trim28 mutant E3.5 embryos
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https://www.ncbi.nlm.nih.gov/sra/SRP090748
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资源简介：
We performed RNAseq on 4 individual E3.5 control and maternal Trim28 blastocysts respectively in order to detect transcriptional differences amog the 2 cohorts which are to be linked to epigentic reprogramming defcts at early stages. Overall design: mRNA isloated from E3.5 embryos was reverse transcribed into cDNA using the SMART-Seq® Ultra® Low Input RNA Kit (Clontech), followed by sequencing library preparation using the Nextera DNA Library Preparation Kit (Illumina) according to manufacturers instructions.
创建时间：
2017-09-17
