Stage-specific RNA-sequencing to identify genes differentially expressed between Toxoplasma gondii tachyzoites and bradyzoites

Using a differentiation reporter strain, unstressed parasites (tachyzoites) or stressed, reporter positive parasites (bradyzoites) were isolated by flow cytometry and analyzed for differential gene expression. Data from three biological replicates. When possible, duplicate samples for each biological replicate and timepoint were taken, libraries prepared in parallel, and treated as technical replicates in downstream analysis