Gene expression data from crypts isolated from WT and Lpcat3 Villin-CreERT2 mice. Mus musculus

We used microarray to analyze the global gene expression in crypts isolated from wild-type (Lpcat3F/F) and Lpcat3 tamoxifen inducible intestine-specific knockout (Lpcat3F/F, Villin-CreERT2) crypts. Phospholipid remodeling is a critical determinant of membrane composition and function. How membrane phospholipid composition affects tissue stem cell function and tumorigenesis is unknown. Here we demonstrate that Lpcat3-dependent phospholipid remodeling regulates intestinal stem cell (ISC) proliferation and promotes tumorigenesis in intestine. The objective of generating this dataset was to analyze the effects of Lpcat3 loss of function on gene expression in mouse intestine crypts. Overall design: Eight- to ten-week old Lpcat3F/F and Lpcat3F/F, Villin-CreERT2 mice were i.p. injected tamoxifen for 5 days (1 mg/mouse/day). Crypts were isolated two weeks post tamoxifen injection. The RNAs were pooled from 5 mice/group and analyzed using Affymetrix Mouse Genome 430A 2.0 Arrays.