X-ray volume (xray2) registered to FIB-SEM of P7 mouse hippocampus (jrc_mus-hippocampus-1)

<br><b>Description</b>: X-ray volume (xray2) registered to FIB-SEM of P7 mouse hippocampus (jrc_mus-hippocampus-1)<b>X-ray ID</b>: xray2<b>Protocol</b>: After re-embedding in Durcupan, a 3D micro-CT scan of the entire block was performed using an XRadia Versa 510 X-ray microscope (Zeiss Microscopy). The tomogram facilitated precise selection of Regions of Interest (ROIs) and defined tissue boundaries. Multiple scans were conducted using different settings, with 4× and 20× objective lenses for overview and ROI selection, respectively. The sample block was then re-mounted on a 1 mm copper post for improved charge dissipation and trimmed to the ROI using an ultramicrotome. After each trimming step, X-ray tomograms were collected. Finally, a thin conductive layer was applied, consisting of a 10 nm gold layer followed by a 100 nm carbon layer.<b>Contributions</b>: X-ray microCT scans and trimming were performed by Zhiyuan Lu (HHMI/Janelia/EMSR). The sample collection through block trimming was supervised by Zhiheng Yu (HHMI/Janelia/EMSR). Regions of interest were selected by Christopher Bleck (HHMI/Janelia/EMSR), Wei-Ping Li (HHMI/Janelia/EMSR), Zhiyuan Lu, and Aubrey Weigel (HHMI/Janelia/CellMap Project Team). Data curation was handled by Rebecca Vorimo (HHMI/Janelia/CellMap Project Team) and Yurii Zubov (HHMI/Janelia/CellMap Project Team). The rest of the CellMap Project Team contributed to the overall development and review of the project. Aubrey Weigel provided oversight for the general project development.<b>X-ray voxel size (nm)</b>:<b>X-ray data dimensions (µm)</b>:<b>Dataset URL</b>: s3://janelia-cosem-datasets/jrc_mus-hippocampus-1/jrc_mus-hippocampus-1.zarr/recon-1/xray/xray-2/<b>Resulting, registered FIB-SEM volume</b>: https://doi.org/10.25378/janelia.24085176<br><br><b>Visualization website</b>: https://openorganelle.janelia.org/datasets/jrc_mus-hippocampus-1

<b>数据集描述</b>: 配准至P7小鼠海马体（jrc_mus-hippocampus-1）聚焦离子束扫描电子显微镜（Focused Ion Beam Scanning Electron Microscopy, FIB-SEM）的X射线体数据（xray2）
<b>X射线数据编号</b>: xray2
<b>实验流程</b>: 经Durcupan包埋剂重新包埋后，使用蔡司显微成像（Zeiss Microscopy）的XRadia Versa 510 X射线显微镜对整个样品块开展三维显微CT扫描。该断层扫描数据可辅助精准选取感兴趣区域（Regions of Interest, ROIs）并明确组织边界。实验采用不同参数设置完成多轮扫描，分别使用4×和20×物镜以实现全景预览与感兴趣区域选取。随后将样品块重新安装至1毫米铜柱上以改善电荷耗散，并使用超薄切片机将样品修整至感兴趣区域范围。每完成一次修整步骤后，均采集X射线断层扫描数据。最终，在样品表面沉积双层薄导电层：先沉积10纳米金层，再沉积100纳米碳层。
<b>贡献人员</b>: X射线显微CT扫描与样品修整工作由陆致远（Zhiyuan Lu，HHMI/Janelia/EMSR）完成。样品块修整流程中的样品收集工作由余志恒（Zhiheng Yu，HHMI/Janelia/EMSR）负责督导。感兴趣区域的选取工作由Christopher Bleck、李伟平（Wei-Ping Li）、陆致远以及Aubrey Weigel（均来自HHMI/Janelia/CellMap项目组）完成。数据管理工作由Rebecca Vorimo与Yurii Zubov（均来自HHMI/Janelia/CellMap项目组）负责。CellMap项目组其余成员参与了项目的整体开发与审核工作。Aubrey Weigel负责项目整体开发的监督工作。
<b>X射线体素尺寸（纳米）</b>:
<b>X射线数据维度（微米）</b>:
<b>数据集链接</b>: s3://janelia-cosem-datasets/jrc_mus-hippocampus-1/jrc_mus-hippocampus-1.zarr/recon-1/xray/xray-2/
<b>最终配准的聚焦离子束扫描电子显微镜体数据</b>: https://doi.org/10.25378/janelia.24085176
<b>可视化网站</b>: https://openorganelle.janelia.org/datasets/jrc_mus-hippocampus-1