Updated Figure S2A-D for - Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision, Phua et al.

Updated Figure S2A-D for "Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision", Phua et al. A link to the original paper: https://www.cell.com/cell/fulltext/S0092-8674(16)31748-2 Figure Legend: (A) Live fluorescence images of Inpp5e+/- MEFs or Inpp5e-/- MEFs expressing 5HT6 -YFP, 5HT6 -YFP-Inpp5e(WT) or 5HT6 -YFP-Inpp5e(PD) with cytosolic mCeru3 respectively after 4 hr of 10% FBS stimulation or at quiescent state (0% FBS). White dashed lines delineate cells. White arrowheads indicate cell- associated YFP+ particles that were likely vesicles released from primary cilia. (B) Quantification of % cells with associated extracellular YFP+ particles under indicated conditions, as in (A). Color coding as in panel above. Data are represented as mean ± SEM. Two-tailed Student’s t tests were performed with p values indicated. (n = 155, 94, 70, 111, 103, 84 cells for respective data from left to right; 3 experiments). (C) Quantification of % ciliation in Inpp5e+/- MEFs expressing 5HT6 -YFP, 5HT6 -YFP-Inpp5e(WT) or 5HT6 -YFP-Inpp5e(PD) with cytosolic mCeru3 at 0 hr and 6 hr of 10% FBS stimulation. Color coding as in panel above. Data are represented as mean ± SEM. Two-tailed Student’s t tests were performed with respect to each 5HT6 -YFP condition, p values indicated. (n = 120, 75, 64, 164, 59, 61 cells for respective data from left to right; 3 experiments). (D) Quantification of % ciliation in Inpp5e-/- MEFs expressing 5HT6 -YFP, 5HT6 -YFP-Inpp5e(WT) or 5HT6 -YFP-Inpp5e(PD) with cytosolic mCeru3 at 0 hr and 6 hr of 10% FBS stimulation. Color coding as in panel above. Data are represented as mean ± SEM. Two-tailed Student’s t tests were performed with respect to each 5HT6 -YFP condition, p values indicated. (n = 138, 97, 77, 148, 87, 80 cells for respective data from left to right; 3 experiments).

本数据集为Phua等发表的论文《Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision》（中文译名为《细胞膜组成动态重塑通过初级纤毛切除驱动细胞周期》）的补充图S2A-D更新版本，原文链接：https://www.cell.com/cell/fulltext/S0092-8674(16)31748-2。图注：(A) 分别转染5HT6-YFP、5HT6-YFP-Inpp5e(野生型，WT)或5HT6-YFP-Inpp5e(PD)并携带胞质定位mCeru3荧光蛋白的Inpp5e基因杂合缺失（Inpp5e+/-）或纯合缺失（Inpp5e-/-）小鼠胚胎成纤维细胞（MEFs），经10%胎牛血清（FBS）刺激4小时后，或处于静息状态（0% FBS）下的活细胞荧光成像图。白色虚线勾勒细胞轮廓，白色箭头指示与细胞结合的YFP阳性颗粒，此类颗粒大概率为初级纤毛释放的囊泡。(B) 参照(A)中的实验设置，对不同处理条件下带有胞外结合YFP阳性颗粒的细胞占比进行定量分析。配色方案与上图一致。数据以平均值±标准误（SEM）表示，采用双侧Student's t检验进行显著性分析，标注对应p值。（从左至右各组细胞数依次为155、94、70、111、103、84；共3次独立实验）(C) 对转染5HT6-YFP、5HT6-YFP-Inpp5e(WT)或5HT6-YFP-Inpp5e(PD)并携带胞质mCeru3的Inpp5e+/- MEFs，在10% FBS刺激0小时和6小时后的纤毛形成率进行定量分析。配色方案与上图一致。数据以平均值±标准误表示，以各组5HT6-YFP为对照进行双侧Student's t检验，标注对应p值。（从左至右各组细胞数依次为120、75、64、164、59、61；共3次独立实验）(D) 对转染5HT6-YFP、5HT6-YFP-Inpp5e(WT)或5HT6-YFP-Inpp5e(PD)并携带胞质mCeru3的Inpp5e-/- MEFs，在10% FBS刺激0小时和6小时后的纤毛形成率进行定量分析。配色方案与上图一致。数据以平均值±标准误表示，以各组5HT6-YFP为对照进行双侧Student's t检验，标注对应p值。（从左至右各组细胞数依次为138、97、77、148、87、80；共3次独立实验）