Additional file 10 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution

Additional file 10: Table S8. qRT-PCR results of Ornithodoros ticks experimentally infected with different ASFV-genotype isolate. Shown are ASFV-P72 transcript-specific Cq-values of third nymphal stage ticks fed with defibrinated pig blood, containing either 1 x 104 HAU/ml or 1 x 106 HAU/ml ASFV-ken.rie1 (GT X) (A-D), 1 x 105 HAU/ml ASFV-Ken06.bus (GT IX) (E-F) or 1 x 104 HAU/ml ASFV-Sardinia (GT I) (G-H). Due to the limited number of field ticks available and feeding under artificial conditions, fifteen Ornithodoros porcinus ticks were collected in each of three experiments and ten in two experiments while for the laboratory-reared Ornithodoros moubata, twenty-five individuals were collected in each of three experiments and fifteen in one experiment. All samples were stored at – 80 °C until RNA-extraction and ASFV transcript-specific qRT-PCR analysis as described in the ‘Material and methods’ section.

附加文件10：表S8。感染不同基因型非洲猪瘟病毒（African Swine Fever Virus, ASFV）分离株的实验钝缘蜱属（Ornithodoros）蜱的实时荧光定量反转录聚合酶链反应（quantitative reverse transcription PCR, qRT-PCR）结果。本表格展示了摄食含不同浓度非洲猪瘟病毒（ASFV）脱纤维猪血的第三若虫期钝缘蜱的ASFV-P72转录本特异性定量循环（quantification cycle, Cq）值：其中猪血分别含有1×10⁴血凝单位（hemagglutinating unit, HAU）/ml或1×10⁶ HAU/ml的ASFV-ken.rie1（基因型X，GT X）（A-D组）、1×10⁵ HAU/ml的ASFV-Ken06.bus（基因型IX，GT IX）（E-F组），以及1×10⁴ HAU/ml的ASFV-Sardinia（基因型I，GT I）（G-H组）。鉴于野外蜱样本数量有限且需人工饲喂，本研究中3组实验各采集15只猪钝缘蜱（Ornithodoros porcinus），2组实验各采集10只；对于实验室饲养的非洲钝缘蜱（Ornithodoros moubata），3组实验各采集25只，1组实验采集15只。所有样本均保存于-80℃，直至按照"Material and methods"部分所述流程进行RNA提取与ASFV转录本特异性qRT-PCR分析。