Effect of in-ovo feeding of iron nanoparticles and methionine hydroxy analogue on broilers chickens small intestinal characteristics

ABSTRACT. The experiment was conducted with 644 Ross fertilized egg by 7 treatments 4 replicates and 23 eggs in each. Seven treatments included two control with and without injection, iron sulfate, iron sulfate nanoparticles, Alimet, Alimet + iron sulfate, Alimet + iron sulfate nanoparticles. After hatching 2 mg iron nanoparticles were applied as new treatment. The highest increased in the intestinal relative weight (p < 0.05) was observed by iron+Alimet in late feeding at day old of age. Also similar trend was found in cecum and duodenum length by iron control 2 and late feeding (18 hours’ after hatching). The highest cecum length was found among all treatments by in ovo injection of iron nanoparticles in early feeding at 21 days of age (p < 0.05). Significantly increased the duodenum length was found by iron sulfate in early feeding at 42 days of age (p < 0.05). In ovo injection of Alimet in late feeding was resulted in decrease jejunum crypt depth at 21 days of age (p < 0.05). The results of this study have shown that the highest jejunum villi width and surface area were recorded in dietary iron sulfate nanoparticles in late feeding at 21 and 42 days of age (p < 0.05).

摘要：本实验以644枚罗斯(Ross)受精蛋为研究对象，设置7个处理组、4个生物学重复，每个重复包含23枚受精蛋。7个处理组分别为：注射与未注射的2个对照组、硫酸亚铁、硫酸亚铁纳米颗粒、阿利梅特(Alimet)、阿利梅特+硫酸亚铁以及阿利梅特+硫酸亚铁纳米颗粒。孵化后施用2 mg铁纳米颗粒作为新增处理组。晚饲阶段，铁+阿利梅特处理组的肠道相对重量增幅最显著（p < 0.05）。盲肠与十二指肠长度指标也呈现出相似趋势：铁对照组2以及孵化后18小时的晚饲组。孵化后21日龄早期饲喂时，卵内注射铁纳米颗粒的处理组盲肠长度为所有组别中最高（p < 0.05）。孵化后42日龄早期饲喂时，硫酸亚铁处理组的十二指肠长度显著增加（p < 0.05）。孵化后21日龄晚饲时，卵内注射阿利梅特可显著降低空肠隐窝深度（p < 0.05）。本研究结果显示，在孵化后21日龄和42日龄的晚饲阶段，日粮添加硫酸亚铁纳米颗粒组的空肠绒毛宽度及表面积均为最高（p < 0.05）。