Genomic data from the Eastern banjo frog Limnodynastes dumerilii dumerilii (Anura: Limnodynastidae)

Amphibian genomes are usually challenging to assemble due to large genome size and high repeat content. The Limnodynastidae is a family of frogs native to Australia, Tasmania and New Guinea. As an anuran lineage that successfully diversified on the Australian continent, it represents an important lineage in the amphibian tree of life but lacks reference genomes. Here we sequenced and annotated the genome of the eastern banjo frog Limnodynastes dumerilii dumerilii to fill this gap. The total length of the genome assembly is 2.38 Gb with a scaffold N50 of 285.9 kb. We identified 1.21 Gb of non-redundant sequences as repetitive elements and annotated 24,548 protein-coding genes in the assembly. BUSCO assessment indicated that more than 94% of the expected vertebrate genes were present in the genome assembly and the gene set. We anticipate that this annotated genome assembly will advance the future study of anuran phylogeny and amphibian genome evolution.

由于两栖动物基因组体量庞大且重复序列占比极高，其基因组组装通常极具挑战性。盾蛙科（Limnodynastidae）是一类自然分布于澳大利亚、塔斯马尼亚与新几内亚的蛙类科群。作为一类在澳大利亚大陆成功完成辐射演化的无尾类（anuran）演化支，该类群在两栖动物生命之树中具有重要研究价值，却始终缺乏对应的参考基因组。本研究针对东部班卓蛙（Limnodynastes dumerilii dumerilii）的基因组开展测序与注释工作，以填补这一研究空白。该基因组组装总长为2.38 Gb，其支架N50（scaffold N50）达285.9 kb。研究团队共鉴定出1.21 Gb的非冗余重复序列，并在该组装序列中注释得到24548个蛋白质编码基因。通用单拷贝同源基因基准测试（BUSCO）评估结果显示，该基因组组装与基因集均覆盖了超过94%的预期脊椎动物保守单拷贝基因。我们预期，这一经过注释的基因组组装结果将推动后续无尾类系统发育与两栖动物基因组演化领域的研究进展。