SYNBIOCHEM Design-Build-Test-Learn pipeline

Quantified titers and peak values measured through different rounds of the SYNBIOCHEM Design-Build-Test-Learn (DBTL) pipeline for microbial production of fine chemicals. The pipeline was applied for the production of flavonoids and alkaloids in Escherichia coli. The flavonoids data contains cinnamate and (2S)-pinocembrin measurements for combinatorial libraries for: a) DBT round 1; b) DBT round 2; c) Chassis selection; d) Media screening; e) Optimization. The alkaloids data provides (S)-reticuline and (S)-scoulerine measurements for a combinatorial library corresponding to one DBTL round.

本数据集包含通过多轮SYNBIOCHEM设计-构建-测试-学习（Design-Build-Test-Learn，DBTL）流程定量测得的微生物精细化学品合成滴度与峰值数据。该流程被应用于大肠杆菌（Escherichia coli）中黄酮类与生物碱类化合物的异源合成。
黄酮类数据集涵盖针对组合文库的肉桂酸与(2S)-松属素（(2S)-pinocembrin）检测数据，对应以下五类场景：a) 第1轮DBT流程；b) 第2轮DBT流程；c) 底盘筛选；d) 培养基筛选；e) 优化环节。
生物碱类数据集则包含对应单轮DBTL流程的组合文库中(S)-网脉番荔枝碱（(S)-reticuline）与(S)-碎叶紫堇碱（(S)-scoulerine）的检测数据。