Raw data of 16-OHPA-Dentinogenesis project

Images: Figure1h(folder): FISH staining of human molar sections, with/without P.gingivalis specific probe (red). DAPI staining for indication nuclear. H&E staining has been taken for illustrating tissue structure. Figure3b.xlsx: MT, Sirius Red and Alizarin Red staining images and data of hODOs with/without 16-OHPA stimulation. Figure3g.xlsx: μCT representative images and data of mouse maxillary molars from reparative dentin secretion model. Figure3h(folder): H&E, MT, IHC staining of mouse maxillary molars from reparative dentin secretion model. Figure5c(folder): p65 IHC staining images of hODOs with/without 16-OHPA stimulation/p65-P1. Figure5f: MT, Sirius Red and Alizarin Red staining images and data of hODOs with/without 16-OHPA stimulation/p65-P1. Figure6c(folder): H3K4me3 IHC staining images of hODOs with/without 16-OHPA stimulation/p65-P1. Figure6d(folder): H3K4me3 IHC staining images of hODOs with/without 16-OHPA stimulation/siIκBζ. Figure7bc(folder): COLLAGEN I(b) and IKBZ(c) IHC staining images of human molar sections. Related data has provided in Figure7def.xlsx. Figures2a.tiff: Alizarin Red staining image of hDPSCs before and after odontogenic inducing. Images of omics analysis were provided separately in PDF form and total omics analysis was packaged and upload to database. Western Blot: Figure5a.xlsx: developing images and data. Figure5d.xlsx: developing images. Figure5f.xlsx: developing images. Figure6ab.xlsx: developing images and data. qPCR: Figure2b.xlsx Figure3de.xlsx Figure5e.xlsx Figure6e.xlsx Figures2b.xlsx Figures5.xlsx Figures3.xlsx: cytotoxicity test of 16-OHPA.图像类数据： 1. 图1h（文件夹）：采用荧光原位杂交（Fluorescence In Situ Hybridization, FISH）对人磨牙组织切片进行染色，分别使用牙龈卟啉单胞菌（Porphyromonas gingivalis, P. gingivalis）特异性探针（标记红色信号），并以4',6-二脒基-2-苯基吲哚（DAPI）染核以标示细胞核位置；同时辅以苏木精-伊红（H&E）染色以展示组织微观结构。 2. Figure3b.xlsx：包含经/不经16-OHPA刺激的人牙本质样成牙本质细胞样细胞（human odontoblast-like cells, hODOs）的马松三色染色（Masson's Trichrome stain, MT）、天狼星红染色、茜素红染色图像及对应实验数据。 3. Figure3g.xlsx：包含修复性牙本质分泌模型中小鼠上颌磨牙的显微计算机断层扫描（micro-computed tomography, μCT）代表性图像及对应实验数据。 4. Figure3h（文件夹）：包含修复性牙本质分泌模型中小鼠上颌磨牙的H&E、MT及免疫组化（Immunohistochemistry, IHC）染色图像。 5. Figure5c（文件夹）：包含经/不经16-OHPA刺激/p65-P1处理的hODOs的p65蛋白免疫组化染色图像。 6. Figure5f：包含经/不经16-OHPA刺激/p65-P1处理的hODOs的MT、天狼星红及茜素红染色图像与对应实验数据。 7. Figure6c（文件夹）：包含经/不经16-OHPA刺激/p65-P1处理的hODOs的组蛋白H3赖氨酸4三甲基化（H3K4me3）免疫组化染色图像。 8. Figure6d（文件夹）：包含经/不经16-OHPA刺激/siIκBζ处理的hODOs的H3K4me3免疫组化染色图像。 9. Figure7bc（文件夹）：包含人磨牙组织切片的I型胶原蛋白（Collagen I, COL I）(b)与免疫球蛋白κBζ（IKBZ）(c)免疫组化染色图像，相关实验数据已在Figure7def.xlsx中提供。 10. Figures2a.tiff：包含人牙髓干细胞（human dental pulp stem cells, hDPSCs）成牙向诱导前后的茜素红染色图像。 11. 组学分析相关图像以PDF格式单独提供，全套组学分析数据集已打包并上传至公共数据库。 蛋白质免疫印迹（Western Blot）类数据： 1. Figure5a.xlsx：包含免疫印迹显影图像及对应定量实验数据。 2. Figure5d.xlsx：包含免疫印迹显影图像。 3. Figure5f.xlsx：包含免疫印迹显影图像。 4. Figure6ab.xlsx：包含免疫印迹显影图像及对应定量实验数据。 实时定量聚合酶链反应（qPCR）类数据： 1. Figure2b.xlsx 2. Figure3de.xlsx 3. Figure5e.xlsx 4. Figure6e.xlsx 5. Figures2b.xlsx 6. Figures5.xlsx Figures3.xlsx：包含16-OHPA的细胞毒性检测实验数据。