Paul Maddox, Aaron Straight, Peg Coughlin, Timothy J. Mitchison, Edward D. Salmon (2011) CIL:13089, Xenopus [NCBITaxon:262014]. CIL. Dataset

Dual-channel spinning-disk confocal time-lapse movie showing kinetochores (Alexa488 labeled CENP-A antibody) in metaphase (red) relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules and kinetochore imaging during metaphase was performed as described in Maddox et al. (2002, 2003), and with a 100X/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. Images were acquired at 5-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other. Movie is video 2 from J Cell Biol 2003 162:377-382.

双通道旋转式盘式共聚焦时间序列电影展示了中期（红色）中着丝粒（标记有Alexa488的CENP-A抗体）相对于极向微管流。如Maddox等人（2002，2003）所述，对中期微管和着丝粒进行了荧光斑点显微镜（FSM）成像。成像使用尼康100X/1.4 NA Plan Apochromat物镜，通过冷却CCD相机（型号ER；Hamamatsu公司）进行2X2合并。MetaMorph®软件（Universal Imaging Corp.）用于控制快门、波长选择、图像采集和存储。图像以5秒间隔采集。不同荧光素序列的连续图像以3-15秒的间隔采集，具体取决于实验。不同颜色通道的成对图像彼此之间相差不超过0.5秒。该电影为《细胞生物学杂志》2003年第162卷第377-382页中的视频2。