Squash preparations and living cells showing chromosome arrangement in mitosis, meiosis I, and meiosis II in green lacewing (Chrysoperla rufilabris) males

In previously-studied species of insects in the order Neuroptera, sex
chromosomes in males exhibit a phenomenon called "distance
segregation," where the X and Y chromosomes are separated and
associated with opposite poles from prometaphase I-anaphase I. This study
aimed to determine the chromosome number and sex determination of a
previously unstudied Neuropteran insect, the green lacewing Chrysoperla
rufilabris (Burmeister, 1839).  Following determination of
chromosome number and sex determination we examined images of fixed and
living specimens to determine if distance segregation was observed. This
dataset includes all of the raw images of living and aceto-orcein squashed
cells that were observed to determine chromosome number, sex
determination, and existence of distance segregation.

针对此前已有研究的脉翅目（Neuroptera）昆虫类群，雄性个体的性染色体存在一种被称为“距离分离（distance segregation）”的现象：X、Y染色体在减数分裂前中期I至后期I阶段发生分离，并分别朝向细胞两极移动。本研究旨在针对一种尚未被研究过的脉翅目昆虫——绿色草蛉（Chrysoperla rufilabris，Burmeister, 1839），明确其染色体数目与性别决定方式。在确定该物种的染色体数目与性别决定方式后，我们对固定标本与活体标本的成像结果进行分析，以验证是否存在距离分离现象。本数据集包含所有用于确定染色体数目、性别决定方式及距离分离现象是否存在的活体细胞与醋酸地衣红（aceto-orcein）压片细胞的原始图像。