CIL:39156, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

采用标准电镜样品制备流程：先以戊二醛固定样本，再经四氧化锇后固定，随后通过酒精梯度脱水并以环氧树脂包埋。以超薄切片机制备厚度约75纳米的组织切片。原始负片通过爱普生Perfection V750 Pro扫描仪完成扫描，所得高分辨率图像最适用于定量分析。更多相关信息可访问：http://www5.pbrc.hawaii.edu/allen/。