BF_BUC_OVS1_NS

To assess changes in immune gene expression following HIV-env vaccination through 3 delivery systems, rhesus macaques were vaccinated with SOSIP trimer with adjuvants using either oral, intraepithelial delivery through a syringe (IEP), a needle free pressurized oral delivery (NF), or an intramuscular injection in the right deltoid (IM). Buccal mucosal samples were collected by cytobrush and stored in Nucleospin lysis buffer RA1 with 1% β-mercaptoethanol and RNA was extracted using Macherey Nagel nucleospin columns. RNA from both timepoints was then used for gene expression analysis using the Nanostring nCounter platform. Data from 6 HIV-env vaccinated adult macaques was obtained from samples representing the 1 week post-vaccination time point