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ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE90663
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The purpose of this experiment was to identify the genes bound by Ndt80 in P. pastoris when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500. P. pastoris Ndt80-myc vs. untagged control, with two technical replicates for each
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2019-05-15
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