RNA sequencing of oocytes at different developmental stages in Astyanax mexicanus

We performed bulk RNA sequencing on different cell size categories to capture different oocyte developmental stages in surface fish, Pachón cavefish and Molino cavefish, allowing us to investigate the differences in reproductive abilities between surface fish and cavefish. Bulk RNA sequencing was performed on right-side ovaries at different cell size categories from age-matched adult surface fish, Pachón cavefish and Molino cavefish at approximately 520 days post fertilization.