dbCAN_annotations.txt

Marsupials exhibit highly specialized patterns of reproduction and development, making them uniquely valuable for comparative genomics studies with their sister lineage, eutherian (also known as placental) mammals. However, marsupial genomic resources still lag far behind those of eutherian mammals, limiting our insight into mammalian diversity. Here, we present a series of novel genomic resources for the fat-tailed dunnart (<em>Sminthopsis crassicaudata</em>), a mouse-like marsupial that, due to its ease of husbandry and <em>ex-utero</em> development, is emerging as a laboratory model. To enable wider use, we have generated a multi-tissue <em>de novo</em> transcriptome assembly of dunnart RNA-seq reads spanning 15 tissues. This highly representative transcriptome is comprised of 2,093,982 assembled transcripts, with a mean transcript length of 830 bp. The transcriptome mammalian BUSCO completeness score of 93% is the highest amongst all other published marsupial transcriptomes. Additionally, we report an improved fat-tailed dunnart genome assembly which is 3.23 Gb long, organized into 1,848 scaffolds, with a scaffold N50 of 72.64 Mb. The genome annotation, supported by assembled transcripts and <em>ab initio</em> predictions, revealed 21,622 protein-coding genes. Altogether, these resources will contribute greatly towards characterizing marsupial biology and mammalian genome evolution.有袋类（Marsupials）展现出高度特化的生殖与发育模式，因此与其姊妹类群——真兽类（eutherian，又称胎盘类）哺乳动物相比，在比较基因组学研究中具备独特的研究价值。然而当前有袋类的基因组资源仍远滞后于真兽类哺乳动物，这限制了我们对哺乳动物多样性的深入认知。本研究针对肥尾狭足袋鼩（<em>Sminthopsis crassicaudata</em>）开发了一系列全新的基因组学资源，这种外形类似小鼠的有袋类动物因易于饲养且可实现体外（ex-utero）发育，正逐渐成为常用的实验室模式生物。为推动该资源的广泛应用，我们基于覆盖15种组织的狭足袋鼩RNA-seq测序数据，构建了多组织从头（de novo）转录组组装。该转录组具有高度的代表性，共包含2,093,982条组装转录本，平均转录本长度为830 bp。其哺乳动物BUSCO完整度达93%，为所有已发表的有袋类转录组中最高水平。此外，本研究还报道了一套优化后的肥尾狭足袋鼩基因组组装版本，其总长度为3.23 Gb，被组装为1,848个支架序列（scaffold），支架N50（scaffold N50）为72.64 Mb。基于组装得到的转录本与从头（ab initio）预测结果进行基因注释，共鉴定出21,622个蛋白质编码基因。综上，这些资源将极大助力我们解析有袋类生物学特性以及哺乳动物基因组演化历程。