Gene expression data from mussels (Mytilus edulis) subjected to stable versus fluctuating temperatures from laboratory experiments conducted in 2023

We measured gene expression levels of blue mussels (Mytilus edulis Linneaus, 1758) under stable versus fluctuating water temperatures. Mussels were obtained from the intertidal zone at Black Point in Narragansett Bay, RI, USA (41° 24′ 4.4964″ N, 71° 27′ 43.8228″ W) and shipped in chilled coolers overnight to the lab at Carleton College in Northfild MN. Mussels were acclimated in a recirculating seawater (Instant Ocean, Blacksburg, VA, USA) tank system (Aquaneering Inc., San Marcos, CA, USA) for 2-3 weeks at 14-16°C and fed commercial Shellfish Diet 1800 (Reed Mariculture, Campbell, CA, USA) at a rate of 5% dry mussel tissue weight day-1

During our experiment, mussels were maintained for five days under one of three stable temperature treatments (e.g., 15, 20, or 25°C) or under a fluctuating temperature treatment between 15 and 25°C. On each day of the experiment, gill tissue from three mussels per temperature treatment were sampled to test for the expression of stress response (hsp70) and cellular respiration (NADH) related genes.

我们针对紫贻贝（Mytilus edulis Linneaus, 1758）开展了恒定与波动水温条件下的基因表达水平测定实验。实验所用个体采集自美国罗德岛州纳拉甘西特湾黑角潮间带（北纬41°24′4.4964″，西经71°27′43.8228″），经冷藏保温箱隔夜运输至美国明尼苏达州诺斯菲尔德卡尔顿学院实验室。
随后将所有紫贻贝置于循环海水养殖系统中驯化2-3周，该系统采用美国弗吉尼亚州布莱克斯堡市Instant Ocean品牌海水产品，配套美国加利福尼亚州圣马科斯市Aquaneering Inc.公司的水箱装置，驯化期间水温控制在14-16℃，每日投喂由美国加利福尼亚州坎贝尔市Reed Mariculture公司生产的商业贝类配合饲料Shellfish Diet 1800，投喂量为贻贝干组织重量的5%每日。
实验阶段，紫贻贝被分为4组培养5天：3组为恒定温度处理组（分别设置为15℃、20℃、25℃），1组为波动温度处理组（水温在15℃至25℃区间波动）。实验期间每日采集各温度处理组的3只紫贻贝的鳃组织，用于检测应激响应相关基因hsp70与细胞呼吸相关基因NADH的表达水平。