Brook trout (Salvelinus fontinalis) cyt b qPCR data from Hidden Lake (Banff National Park, Canada) over two rotenone applications between 2018 and 2020.

Water samples were taken in Hidden Lake at five different time points around two rotenone applications: (i) five weeks prior to the first rotenone application, on July 12 2018; (ii) approximately three weeks after the first application of rotenone, on 7 September 2018; (iii) approximately 10 months after the first rotenone application, on 10 July 2019; and (iv) one year after the final rotenone treatment, on 19 August 2020. For each time point, four pelagic and four littoral water samples were taken from Hidden Lake, as well as 8 to 13 water samples from Hidden Creek and Coral Creek for a total of 16 to 21 samples per time point. Quantitative PCR (qPCR) method was used to produce brook trout (Salvelinus fontinalis) cytochrome b copy number for each sample. The objective of this study was use eDNA to assess the efficacy of invasive brook trout removal using rotenone.

本研究在隐湖（Hidden Lake）围绕两次鱼藤酮（rotenone）施药流程设置了5个不同采样时间点采集水样：(i) 首次鱼藤酮施药前5周，即2018年7月12日；(ii) 首次鱼藤酮施药后约3周，即2018年9月7日；(iii) 首次鱼藤酮施药后约10个月，即2019年7月10日；(iv) 末次鱼藤酮处理后1年，即2020年8月19日。每个采样时间点下，研究人员从隐湖采集4份浮游水样与4份沿岸带水样，同时从隐溪（Hidden Creek）与珊瑚溪（Coral Creek）采集8至13份水样，单个时间点总采样量为16至21份。采用实时定量聚合酶链式反应（quantitative PCR，qPCR）方法，对每份样品中的溪红点鲑（Salvelinus fontinalis）细胞色素b基因拷贝数进行定量检测。本研究旨在利用环境DNA（environmental DNA，eDNA）评估鱼藤酮去除入侵性溪红点鲑的效果。