Impact of smoking cessation, coffee and bread consumption on the intestinal microbial composition among Saudis: A cross-sectional study

The gut microbiota is often affected by the dietary and lifestyle habits of the host, resulting in a better efficacy that favors energy harvesting from the consumed food. Our objective was to characterize the composition of gut microbiota in adult Saudis and investigate possible association with lifestyle and dietary practices. Feces from 104 Saudi volunteers (48% males) were tested for microbiota by sequencing the V3-V4 region of bacterial 16S ribosomal RNA (rRNA). For all participants, data were collected related to their lifestyle habits and dietary practices. The relative abundance (RA) of Fusobacteria was significantly higher in normal weight Saudis ( p =0.005, FDR=0.014). Individuals who consumed more coffee presented marginally significant more RA of Fusobacteria ( p =0.02, FDR=0.20) in their gut microbiota compared to those reporting low or no coffee intake, but the RA of Fusobacteria was significantly higher in smokers compared to non-smokers p =0.009, FDR=0.027) . The RA...

肠道菌群（gut microbiota）常受宿主饮食与生活习惯影响，可提升宿主从摄入食物中获取能量的效率。本研究旨在明确成年沙特人群肠道菌群的组成特征，并探究其与生活方式及饮食行为的潜在关联。本研究对104名沙特志愿者（男性占比48%）的粪便样本，通过对细菌16S核糖体RNA（rRNA）V3-V4区测序以检测其肠道菌群组成；同时收集所有受试者的生活习惯与饮食行为相关数据。梭杆菌门（Fusobacteria）的相对丰度（relative abundance，RA）在体重正常的沙特人群中显著更高（p=0.005，错误发现率（false discovery rate，FDR）=0.014）。与咖啡摄入较少或无咖啡摄入的个体相比，咖啡摄入较多的个体肠道内梭杆菌门的相对丰度差异具有边缘显著性（p=0.02，FDR=0.20）；而吸烟者肠道内梭杆菌门的相对丰度显著高于非吸烟者（p=0.009，FDR=0.027）。其相对丰度（RA）……