Magnetic control of GFP-like fluorescent proteins - Raw data 1

We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetoresponsive in the presence of an appropriate cofactor (e.g. EGFP-FlavinTag, or an mScarlet/FMN solution). This method works at room-temperature and body-temperature, in vitro, in E. coli and in cultured mammalian cells. The GFP-family magnetoresponse is weak (ΔF/F≈1%), but shows the hallmarks of evolvability. This suggests exciting technological possibilities, both short-term (e.g. lock-in detection, multiplexing) and long-term (e.g. optically-detected MRI, magnetogenetics). We've also discovered weak magnetoresponse from a member of the LOV-domain family. This suggests the possibility that magnetoresponse is a general feature of fluorescent proteins, and not unique to the cryptochrome/photolyase family. This repository holds some of the raw data for the main text figures. For the contents of the paper, please see: doi.org/10.5281/zenodo.8137174本研究发现了一种简便、无毒且生物相容的调控方法，可通过适度磁场（约10 mT）调控类绿色荧光蛋白（GFP-like fluorescent proteins）的发光亮度。原本无磁响应特性的荧光蛋白（如EGFP、mScarlet）在适配辅因子存在时可获得磁响应性能，例如EGFP-FlavinTag融合蛋白或mScarlet/FMN溶液。该方法可在室温与体温条件下，于体外环境、大肠杆菌（E. coli）及培养的哺乳动物细胞中生效。 GFP家族蛋白的磁响应强度较弱，其荧光强度相对变化量（ΔF/F≈1%），但具备可演化性的典型特征。这一发现带来了诸多极具潜力的技术应用方向：短期可应用于锁相检测、多路复用等场景，长期则可用于光学检测磁共振成像（MRI）、磁遗传学等领域。 本研究同时在光-氧-电压结构域（LOV-domain）家族的成员中发现了微弱的磁响应现象，这表明磁响应或许是荧光蛋白的通用特性，而非仅局限于隐花色素/光解酶家族。 本数据集仓库包含了论文正文图表所用的部分原始数据。若需获取完整论文内容，请访问：doi.org/10.5281/zenodo.8137174