Cortical single-cell RNA sequencing of the microglia specific TREM2 inducible mouse models in the early stage of amyloid deposition

We induced TREM2 expression at birth in our 5xFAD/TREM2 mouse models and harvested the animals at 3.5 months of age. The single cells from the cortex were isolated and subjected to the single-cell RNA seq to investigate the effects of TREM2 on microglia transcriptomic profiles in the early stage of amyloid development. Our data showed that TREM2-WT, to a lesser extent of TREM2-R47H, restricted the disease associated microglia (DAM) signature at this stage. Overall design: Six mice (3 males and 3 females) were analyzed for each of the four groups, including 5xFAD/TREM2-WT-Ctrl, 5xFAD/TREM2-WT-Induced, 5xFAD/TREM2-R47H-Ctrl, 5xFAD/TREM2-R47H-Induced .