The RNA expression level of each gene in samples left on the transfer catheters

Samples left on the transfer catheter after cleavage stage embryos transfer (day 3, D3, presumable pre-receptivity, n=96) and blastocysts transfer (day 5, D5, presumable receptivity, n=127) in 223 ART patients who achieved successful clinical pregnancies subsequently were collected. The RNA expression level of each gene was estimated by FPKM (fragments per kilobase million).

本研究收集了223例后续获得成功临床妊娠的辅助生殖技术（Assisted Reproductive Technology, ART）患者的移植导管残留样本，这些样本分别取自卵裂期胚胎（第3天，D3，推定着床前非接受态，n=96）移植与囊胚（第5天，D5，推定着床接受态，n=127）移植。随后采用每千碱基百万片段数（FPKM，fragments per kilobase million）对每个基因的RNA表达水平进行估算。