RNA-expression profiling of LMP1 expressing mouse B cells before and after transformation in vivo

Non-transformed LMP1 expressing mouse B cells or mouse LMP1 lymphomas were analysed by RNA-sequencing Overall design: 14 Rag2KOcgKO were reconstituted with fetal liver hematopoietic stem and progenitor cells (HSPC) from Cd3eKO Cd19-Cre R26LMP1stopf mice. From 3 mice LMP1 expressing non-transformed B cells were isolated day 18-20 post reconstitution. 11 mice developed LMP1-driven B-cell lymphomas. Thse lymphomas were transplanted into Rag2KOcgKO or Rag2KO mice. Arising secondary lymphomas were FACSpurified (CD19+huCD2+). RNA of sorted lymphoma samples was analysed by RNA sequencing.