Enzymatic activities of wild-type and mutant φ29 DNA polymerases.

aNumbers indicate the dNTP concentration (in nM) required to efficiently elongate the 15mer primer until the 20mer position.b15mer/21mer hybrid molecule.cData represent the percent activity with respect to the wild-type DNA polymerase (100%) and the standard deviation obtained from at least three independent experiments.dKm stands for Michaelis Mentent constant for the incoming nucleotide (in nM). Data are shown as Mean ± S.D. corresponding to four independent measurements.a 本数值代表可高效将15聚体引物（15mer primer）延伸至20聚体位置所需的脱氧核糖核苷三磷酸（dNTP）浓度，单位为纳摩尔（nM）。 b 15聚体/21聚体杂交分子（15mer/21mer hybrid molecule）。 c 本数据为相对于野生型DNA聚合酶（活性设定为100%）的活性百分比，以及至少3次独立实验所得的标准差。 d Km代表掺入核苷酸的米氏常数（Michaelis-Menten constant，单位为nM）。数据以平均值±标准差（Mean ± S.D.）形式呈现，对应4次独立测量结果。