Tip60-mediated H2A.Z acetylation is required for neuronal fate specification and bivalent gene activation [RNA-Seq]

Examination of the effects of Tip60/Kat5 depletion on gene expression dynamics during Ascl1-mediated direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced neuronal cells. Overall design: Gene expression (RNAseq) of (i) MEFs transduced with shRNA targeting Tip60 (shTip60) or non-targeting control shRNA (shNTC) at days 4 and 7 post-infection, and (ii) rtTA control or Ascl1-transduced MEFs co-expressing shTip60 or shNTC at days 2 and 5 of iN cell reprogramming (corresponding to days 4 and 7 post-infection, respectively).