The bacterial metagenome of Schizaphis graminum

The bacterial metagenome in greenbug (Schizaphis graminum) aphids was investigated in response to aphid biotype, host species, host cultivar, and time post infestation. The V5-V7 hypervariable region from 16S rRNA genes was amplified from whole aphids, sequenced, and characterized against bacterial 16S sequences. Greenbug biotypes E and K were placed on potted plants of 17 accessions/cultivars in growth chambers. The accessions were: A1, Aegilops triuncialis TA1675 (Gbz), A2, Ae. triuncialis TA1695 (Gbx1); B1 to B2, barley cultivars Wintermalt, Jao (RSg1), and Post90; R1 and R2, rye cultivars Elbon and Insave F.A. (Gb2 amd Gb6); S1 to S4, sorghum cultivars TX7000, TX2737, TX2783, and DZhugara Belaya (PI 550607); and W1 through W6, wheat cultivars Newton, Custer, Amigo, Largo, C117882, and GRS 1201. Whole aphids were sampled by freezing in liquid nitrogen immediately before placement and 2, 4, and 8 days after placement. DNA was extracted using the DNeasy Blood and Tissue Kit for DNA Isolation (Qiagen, Germantown, MD) following its instructions. PCR was performed with V5-V7 primers 799F (degenerate AACMGGATTAGATACCCKG) and 1193R (ACGTCATCCCCACCTTCC). The libraries were barcoded, pooled, and sequenced on an Illumina Novaseq to generate 2x250-base paired-end reads. Demultiplexed reads were returned as fastq files with the biotype, host species, host cultivar, time point, and replicate encoded in each file name as BSC_TR, where B is biotype E or K; S is A (Aegilops triuncialis), B (barley), R (rye), S (sorghum), or W (wheat); C is a number from 1 to 6 for the cultivar as listed above; T is a number from 0 to 3 as 0, 2, 4, or 8 days; and R is biological replicate 1 to 3. This data set adds to knowledge of bacterial microfloras in the group of related genera (Aphis, Rhopalosiphum) to which Schizaphis belongs.

本研究针对绿盲蝽（Schizaphis graminum）蚜虫的细菌宏基因组进行了研究，以响应蚜虫生物型、宿主物种、宿主品种以及侵染后时间等因素。通过对16S rRNA基因的V5-V7高变区进行扩增、测序，并将其与细菌16S序列进行特征描述。将绿盲蝽生物型E和K置于生长室中的17个品种的盆栽植物上。品种包括：A1，Aegilops triuncialis TA1675（Gbz），A2，Ae. triuncialis TA1695（Gbx1）；B1至B2，冬大麦品种Wintermalt，Jao（RSg1），以及Post90；R1和R2，黑麦品种Elbon和Insave F.A.（Gb2和Gb6）；S1至S4，高粱品种TX7000，TX2737，TX2783，以及DZhugara Belaya（PI 550607）；以及W1至W6，小麦品种Newton，Custer，Amigo，Largo，C117882，和GRS 1201。在放置前立即使用液氮冷冻整只蚜虫进行采样，并在放置后2天、4天和8天进行采样。按照DNeasy Blood and Tissue Kit for DNA Isolation（Qiagen，Germantown，MD）的说明提取DNA。使用V5-V7引物799F（退化的AACMGGATTAGATACCCKG）和1193R（ACGTCATCCCCACCTTCC）进行PCR扩增。对文库进行条形码标记、混合，并在Illumina Novaseq上进行测序，生成2x250碱基的配对末端读数。解复用后的读数以fastq文件形式返回，文件名中包含生物型、宿主物种、宿主品种、时间点和重复编码，格式为BSC_TR，其中B表示生物型E或K；S表示A（Aegilops triuncialis）、B（大麦）、R（黑麦）、S（高粱）或W（小麦）；C为上述品种列表中的1至6的数字；T为0至3的数字，代表0、2、4或8天；R为生物重复1至3。该数据集丰富了关于Schizaphis所属的与相关属（Aphis，Rhopalosiphum）相关的细菌微生物群的认知。